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  • Featured Product
  • KD/KO Validated

RARB Polyclonal antibody, PBS Only

RARB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14013-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RARB Polyclonal antibody (14013-1-AP)

    WB analysis using 14013-1-AP (same clone as 14013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14013-1-AP (RARB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse bladder using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat bladder tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of rat bladder using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of rat brain using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of human pancreas cancer using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of human renal cell carcinoma using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse brain using 14013-1-AP (same clone as 14013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RARB Polyclonal antibody (14013-1-AP)

    IF Staining of MCF-7 using 14013-1-AP (same clone as 14013-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RARB antibody (14013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14013-1-PBS targets RARB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RARB fusion protein Ag5119 相同性解析による交差性が予測される生物種
Full Name retinoic acid receptor, beta
Calculated molecular weight 50 kDa
Observed molecular weight37-51 kDa
GenBank accession numberBC060794
Gene symbol RARB
Gene ID (NCBI) 5915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).