RASGRF1 Monoclonal antibody
RASGRF1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
rat, pig, Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A4D4
Cat no : 67010-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig cerebellum tissue, pig brain tissue, rat brain tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67010-1-Ig targets RASGRF1 in WB, ELISA applications and shows reactivity with rat, pig, Human samples.
| Tested Reactivity | rat, pig, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RASGRF1 fusion protein Ag6937 相同性解析による交差性が予測される生物種 |
| Full Name | Ras protein-specific guanine nucleotide-releasing factor 1 |
| Calculated molecular weight | 134 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC040275 |
| Gene symbol | RASGRF1 |
| Gene ID (NCBI) | 5923 |
| RRID | AB_2882327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RASGRF1 antibody 67010-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
