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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASGRP2 Polyclonal antibody

RASGRP2 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 30189-1-AP

Synonyms

GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RASGRP2 Polyclonal antibody (30189-1-AP)

    WB analysis using 30189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30189-1-AP (RASGRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using RASGRP2 Polyclonal antibody (30189-1-AP)

    FC experiment of Jurkat using 30189-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human RASGRP2 (30189-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells, Raji cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30189-1-AP targets RASGRP2 in WB, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGRP2 fusion protein Ag32762 相同性解析による交差性が予測される生物種
Full Name RAS guanyl releasing protein 2 (calcium and DAG-regulated)
Calculated molecular weight69kd
Observed molecular weight69 kDa
GenBank accession numberNM_153819
Gene symbol RASGRP2
Gene ID (NCBI) 10235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.

Protocols

Product Specific Protocols
WB protocol for RASGRP2 antibody 30189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Biochim Biophys Acta Mol Cell Res

NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras

Authors - Guozhu Chen
View Article