CoraLite® Plus 488-conjugated RASGRP2 Polyclonal antibody
RASGRP2 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-30189
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Jurkat cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-30189 targets RASGRP2 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RASGRP2 fusion protein Ag32762 相同性解析による交差性が予測される生物種 |
| Full Name | RAS guanyl releasing protein 2 (calcium and DAG-regulated) |
| Calculated molecular weight | 69kd |
| Observed molecular weight | 69 kDa |
| GenBank accession number | NM_153819 |
| Gene symbol | RASGRP2 |
| Gene ID (NCBI) | 10235 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 RASGRP2 antibody CL488-30189 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
