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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASGRP3 Monoclonal antibody

RASGRP3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10G4

Cat no : 66650-1-Ig

Synonyms

GRP3, KIAA0846, Ras guanyl releasing protein 3, RASGRP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RASGRP3 Monoclonal antibody (66650-1-Ig)

    WB analysis using 66650-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RASGRP3 Monoclonal antibody (66650-1-Ig)

    WB analysis of HEK-293 using 66650-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RASGRP3 Monoclonal antibody (66650-1-Ig)

    WB analysis of Jurkat using 66650-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RASGRP3 Monoclonal antibody (66650-1-Ig)

    IF Staining of HEK-293 using 66650-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inRaji cells, HEK-293 cells, Jurkat cells, Ramos cells, Raw 264.7 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66650-1-Ig targets RASGRP3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RASGRP3 fusion protein Ag3810 相同性解析による交差性が予測される生物種
Full Name RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Calculated molecular weight 690 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC027849
Gene symbol RASGRP3
Gene ID (NCBI) 25780
RRIDAB_2882007
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

Protocols

Product Specific Protocols
WB protocol for RASGRP3 antibody 66650-1-IgDownload protocol
IF protocol for RASGRP3 antibody 66650-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,