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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RASGRP3 Monoclonal antibody (66650-1-Ig)

    WB analysis using 66650-1-Ig (same clone as 66650-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RASGRP3 Monoclonal antibody (66650-1-Ig)

    WB analysis of HEK-293 using 66650-1-Ig (same clone as 66650-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RASGRP3 Monoclonal antibody (66650-1-Ig)

    WB analysis of Jurkat using 66650-1-Ig (same clone as 66650-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RASGRP3 Monoclonal antibody (66650-1-Ig)

    IF Staining of HEK-293 using 66650-1-Ig (same clone as 66650-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

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RASGRP3 Monoclonal antibody, PBS Only

RASGRP3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66650-1-PBS
Clone No.1A10G4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66650-1-PBS targets RASGRP3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RASGRP3 fusion protein Ag3810 相同性解析による交差性が予測される生物種
Full Name RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Calculated molecular weight 690 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC027849
Gene Symbol RASGRP3
Gene ID (NCBI) 25780
RRIDAB_2882007
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IV61
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).