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KD/KO Validated

RB1CC1 Polyclonal antibody

Name: RB1CC1 Polyclonal antibody
Brand: Proteintech
SKU: 17250-1-AP
Rating: 5.00 (1 reviews)

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

103

Reviews (1)

Cat no : 17250-1-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 17250-1-AP
WB result of RB1CC1 antibody (17250-1-AP, 1:2000) with si-Control and si-RB1CC1 transfected HEK293 cells.
WB analysis of HEK-293 using 17250-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 17250-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17250-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17250-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17250-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17250-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17250-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17250-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17250-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 17250-1-AP
IP result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 2500ug.
IP experiment of HEK-293 using 17250-1-AP
IP result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 1600ug.
IHC staining of mouse brain using 17250-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17250-1-AP (RB1CC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17250-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17250-1-AP (RB1CC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17250-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 17250-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 17250-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 17250-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 17250-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RB1CC1 antibody (17250-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Cat No. 17250-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 20 publications below
WB	See 73 publications below
IHC	See 5 publications below
IF	See 32 publications below
IP	See 2 publications below
CoIP	See 3 publications below

Product Information

17250-1-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RB1CC1 fusion protein Ag10508 相同性解析による交差性が予測される生物種
Full Name	RB1-inducible coiled-coil 1
Calculated molecular weight	1594 aa, 183 kDa
Observed molecular weight	200 kDa
GenBank accession number	BC017556
Gene symbol	RB1CC1
Gene ID (NCBI)	9821
RRID	AB_10666428
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Protocols

Product Specific Protocols
WB protocol for RB1CC1 antibody 17250-1-AP	Download protocol
IHC protocol for RB1CC1 antibody 17250-1-AP	Download protocol
IF protocol for RB1CC1 antibody 17250-1-AP	Download protocol
IP protocol for RB1CC1 antibody 17250-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival Authors - Pauline Lascaux View Article
human	IF	Cell Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes Authors - Suresh Kumar View Article
human	IF	Cell CCT2 is an aggrephagy receptor for clearance of solid protein aggregates. Authors - Xinyu Ma View Article
mouse	WB	Nat Immunol Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense. Authors - Miwa Sasai View Article
human	WB,IF	Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. Authors - Shulin Li KD Validated View Article
human	WB	Nat Cell Biol The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Authors - Andrew M Leidal KO Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

SANGAPPA (Verified Customer) (01-04-2020)

Works very good in western blotting!

Applications: Western Blot,
Primary Antibody Dilution: 1/1000
Cell Tissue Type: Human endometrial stromal cells

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 17250-1-AP

WB analysis of HEK-293 using 17250-1-AP

WB analysis using 17250-1-AP

WB analysis of K-562 using 17250-1-AP

WB analysis of Jurkat using 17250-1-AP

WB analysis of Jurkat using 17250-1-AP

WB analysis of HEK-293 using 17250-1-AP

WB analysis of HEK-293 using 17250-1-AP

WB analysis of MCF-7 using 17250-1-AP

WB analysis of MCF-7 using 17250-1-AP

IP experiment of HEK-293 using 17250-1-AP

IP experiment of HEK-293 using 17250-1-AP

IHC staining of mouse brain using 17250-1-AP

IHC staining of mouse brain using 17250-1-AP

IHC staining of human liver cancer using 17250-1-AP

IHC staining of human breast cancer using 17250-1-AP

IHC staining of human breast cancer using 17250-1-AP

IHC staining of human liver cancer using 17250-1-AP

IF Staining of HepG2 using 17250-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.

Reviews

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