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CoraLite® Plus 488-conjugated RB1CC1 Polyclonal antibody
RB1CC1 Polyclonal Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-17250
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-17250 targets RB1CC1 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RB1CC1 fusion protein Ag10508 相同性解析による交差性が予測される生物種 |
| Full Name | RB1-inducible coiled-coil 1 |
| Calculated molecular weight | 1594 aa, 183 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC017556 |
| Gene symbol | RB1CC1 |
| Gene ID (NCBI) | 9821 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 RB1CC1 antibody CL488-17250 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
