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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated RBAP48 Monoclonal antibody

RBAP48 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

5C4D6

Cat no : CL555-66060

Synonyms

RBBP4, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48, CAF-I 48 kDa subunit

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated RBAP48 Monoclonal antibody (CL555-66060)

    IF Staining of HepG2 using CL555-66060

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 RBAP48 antibody (CL555-66060, Clone: 5C4D6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite®555-conjugated RBAP48 Monoclonal antibody (CL555-66060)

    FC experiment of A549 using CL555-66060

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human RBAP48 (CL555-66060, Clone:5C4D6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66060 targets RBAP48 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RBAP48 fusion protein Ag6196 相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 4
Calculated molecular weight 48 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC053904
Gene symbol RBBP4
Gene ID (NCBI) 5928
RRIDAB_2919671
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

Protocols

Product Specific Protocols
IF protocol for CL555 RBAP48 antibody CL555-66060Download protocol
FC protocol for CL555 RBAP48 antibody CL555-66060Download protocol
Standard Protocols
Click here to view our Standard Protocols