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KD/KO Validated

RBM15 Polyclonal antibody

Name: RBM15 Polyclonal antibody
Brand: Proteintech
SKU: 10587-1-AP

RBM15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10587-1-AP

Synonyms

RNA-binding protein 15, RNA-binding motif protein 15, RBM 15, OTT1, OTT

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of HEK293 cells using 10587-1-AP
WB result of RBM15 antibody (10587-1-AP, 1:3000) with si-control and si-RBM15 transfected HEK293 cells.
WB analysis of HEK-293 using 10587-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 10587-1-AP
SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 10587-1-AP
SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10587-1-AP
IP result of anti-RBM15 (IP:10587-1-AP, 4ug; Detection:10587-1-AP 1:500) with HEK-293 cells lysate 2600 ug.
IHC staining of human breast cancer using 10587-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10587-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 10587-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM15 antibody (10587-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 10587-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM15 antibody (10587-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Cat No. 10587-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SW480 cells, HEK293 cells, HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 38 publications below
IHC	See 11 publications below
IF	See 6 publications below
IP	See 4 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

Product Information

10587-1-AP targets RBM15 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RBM15 fusion protein Ag0938 相同性解析による交差性が予測される生物種
Full Name	RNA binding motif protein 15
Calculated molecular weight	105 kDa
Observed molecular weight	100-107 kDa
GenBank accession number	BC006397
Gene symbol	RBM15
Gene ID (NCBI)	64783
RRID	AB_2175757
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Protocols

Product Specific Protocols
WB protocol for RBM15 antibody 10587-1-AP	Download protocol
IHC protocol for RBM15 antibody 10587-1-AP	Download protocol
IF protocol for RBM15 antibody 10587-1-AP	Download protocol
IP protocol for RBM15 antibody 10587-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature METTL3 regulates heterochromatin in mouse embryonic stem cells. Authors - Wenqi Xu KO Validated View Article
human	WB,IF	Mol Cancer Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer Authors - Yufan Ying View Article
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa View Article
human	WB,IF,RIP,CoIP	Dev Cell N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles. Authors - Xinyu Wang KD Validated View Article
human	WB	Elife Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. Authors - Li Zhang View Article
human	WB,IF,IP	Nucleic Acids Res Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Authors - Zolotukhin Andrei S AS View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK293 cells using 10587-1-AP

WB analysis of HEK-293 using 10587-1-AP

WB analysis of SW480 using 10587-1-AP

WB analysis of SW480 using 10587-1-AP

IP experiment of HEK-293 using 10587-1-AP

IHC staining of human breast cancer using 10587-1-AP

IHC staining of human breast cancer using 10587-1-AP

IF Staining of A431 using 10587-1-AP

IF Staining of HepG2 using 10587-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.