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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10587

Synonyms

FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RBM15 Polyclonal antibody (CL594-10587)

    IF Staining of HepG2 using CL594-10587

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM15 antibody (CL594-10587) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10587 targets RBM15 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM15 fusion protein Ag0938 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 15
Calculated molecular weight 105 kDa
Observed molecular weight 100-107 kDa
GenBank accession numberBC006397
Gene symbol RBM15
Gene ID (NCBI) 64783
RRIDAB_2919738
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Protocols

Product Specific Protocols
IF protocol for CL594 RBM15 antibody CL594-10587Download protocol
Standard Protocols
Click here to view our Standard Protocols