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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RBM15 Monoclonal antibody

RBM15 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4A1A4

Cat no : CL594-66059

Synonyms

RNA-binding motif protein 15, RBM 15, OTT1, OTT, One-twenty two protein 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated RBM15 Monoclonal antibody (CL594-66059)

    IF Staining of HeLa using CL594-66059

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 RBM15 antibody (CL594-66059, Clone: 4A1A4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated RBM15 Monoclonal antibody (CL594-66059)

    FC experiment of HeLa using CL594-66059

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM15 (CL594-66059, Clone:4A1A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66059 targets RBM15 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBM15 fusion protein Ag17970 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 15
Calculated molecular weight 105 kDa
Observed molecular weight 107 kDa
GenBank accession numberBC006397
Gene symbol RBM15
Gene ID (NCBI) 64783
RRIDAB_2919943
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Protocols

Product Specific Protocols
IF protocol for CL594 RBM15 antibody CL594-66059Download protocol
FC protocol for CL594 RBM15 antibody CL594-66059Download protocol
Standard Protocols
Click here to view our Standard Protocols