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RBP4 Monoclonal antibody

RBP4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66104-1-Ig
Clone No.1D12B11

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  150 μL, 2100 μg/ml
20 μL, 2100 μg/ml
150 μL, 2100 μg/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inHuman Blood, tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66104-1-Ig targets RBP4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitypig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBP4 fusion protein Ag19295 相同性解析による交差性が予測される生物種
Full Name retinol binding protein 4, plasma
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC020633
Gene Symbol RBP4
Gene ID (NCBI) 5950
RRIDAB_2881503
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Protocols

Product Specific Protocols
WB protocol for RBP4 antibody 66104-1-IgDownload protocol
IHC protocol for RBP4 antibody 66104-1-IgDownload protocol
IF protocol for RBP4 antibody 66104-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Commun Biol

Retinol binding protein 4 restricts PCV2 replication via selective autophagy degradation of viral ORF1 protein

Authors - Qingbing Han
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