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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBP4 Monoclonal antibody, PBS Only

RBP4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D12B11

Cat no : 66104-1-PBS

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using RBP4 Monoclonal antibody (66104-1-Ig)

    WB analysis of human plasma using 66104-1-Ig (same clone as 66104-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66104-1-Ig (RBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBP4 Monoclonal antibody (66104-1-Ig)

    IHC staining of human liver cancer using 66104-1-Ig (same clone as 66104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBP4 Monoclonal antibody (66104-1-Ig)

    IHC staining of human liver cancer using 66104-1-Ig (same clone as 66104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RBP4 Monoclonal antibody (66104-1-Ig)

    IHC staining of human liver using 66104-1-Ig (same clone as 66104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RBP4 Monoclonal antibody (66104-1-Ig)

    IHC staining of human liver using 66104-1-Ig (same clone as 66104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using RBP4 Monoclonal antibody (66104-1-Ig)

    IF Staining of human liver cancer using 66104-1-Ig (same clone as 66104-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBP4 Monoclonal antibody (66104-1-Ig)

    IF Staining of HepG2 using 66104-1-Ig (same clone as 66104-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66104-1-PBS targets RBP4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBP4 fusion protein Ag19295 相同性解析による交差性が予測される生物種
Full Name retinol binding protein 4, plasma
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC020633
Gene symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)