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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F4D9

Cat no : CL594-66716

Synonyms

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated RBX1 Monoclonal antibody (CL594-66716)

    IF Staining of HeLa using CL594-66716

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®®594 RBX1 antibody (CL594-66716, Clone: 1F4D9 ) at dilution of 1:100. DAPI (blue).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66716 targets RBX1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBX1 fusion protein Ag7005 相同性解析による交差性が予測される生物種
Full Name ring-box 1
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC001466
Gene symbol RBX1
Gene ID (NCBI) 9978
RRIDAB_2920026
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

Protocols

Product Specific Protocols
IF protocol for CL594 RBX1 antibody CL594-66716Download protocol
Standard Protocols
Click here to view our Standard Protocols