Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse brain tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67335-1-Ig targets RCC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RCC1 fusion protein Ag17809 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of chromosome condensation 1 |
| Calculated molecular weight | 421 aa, 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC010067 |
| Gene Symbol | RCC1 |
| Gene ID (NCBI) | 1104 |
| RRID | AB_2882593 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P18754 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RCC1 antibody 67335-1-Ig | Download protocol |
| IHC protocol for RCC1 antibody 67335-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |