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RCC1 Monoclonal antibody

RCC1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67335-1-Ig
Clone No.2C2F5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67335-1-Ig targets RCC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RCC1 fusion protein Ag17809 相同性解析による交差性が予測される生物種
Full Name regulator of chromosome condensation 1
Calculated molecular weight 421 aa, 45 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_2882593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 67335-1-IgDownload protocol
IHC protocol for RCC1 antibody 67335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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