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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RCC1 Monoclonal antibody, PBS Only

RCC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C2F5

Cat no : 67335-1-PBS

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RCC1 Monoclonal antibody (67335-1-Ig)

    WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of human stomach cancer using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of human stomach cancer using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67335-1-PBS targets RCC1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RCC1 fusion protein Ag17809 相同性解析による交差性が予測される生物種
Full Name regulator of chromosome condensation 1
Calculated molecular weight 421 aa, 45 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC010067
Gene symbol RCC1
Gene ID (NCBI) 1104
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.