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  • Featured Product
  • KD/KO Validated

RCHY1 Monoclonal antibody

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C11C11

Cat no : 67856-1-Ig

Synonyms

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RCHY1 Monoclonal antibody (67856-1-Ig)

    WB analysis of HEK-293 using 67856-1-Ig

    WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using RCHY1 Monoclonal antibody (67856-1-Ig)

    WB analysis using 67856-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCHY1 Monoclonal antibody (67856-1-Ig)

    IHC staining of human breast cancer using 67856-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCHY1 Monoclonal antibody (67856-1-Ig)

    IHC staining of human breast cancer using 67856-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RCHY1 Monoclonal antibody (67856-1-Ig)

    IF Staining of HeLa using 67856-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RCHY1 Monoclonal antibody (67856-1-Ig)

    IF Staining of A549 using 67856-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, Daudi cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67856-1-Ig targets RCHY1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RCHY1 fusion protein Ag4942 相同性解析による交差性が予測される生物種
Full Name ring finger and CHY zinc finger domain containing 1
Calculated molecular weight 261 aa, 30 kDa
GenBank accession numberBC047393
Gene symbol RCHY1
Gene ID (NCBI) 25898
RRIDAB_2918614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

Protocols

Product Specific Protocols
WB protocol for RCHY1 antibody 67856-1-IgDownload protocol
IHC protocol for RCHY1 antibody 67856-1-IgDownload protocol
IF protocol for RCHY1 antibody 67856-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock

Authors - Sharanya Nag
  • KD Validated
View Article