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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RCHY1 Monoclonal antibody (67856-1-Ig)

    WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RCHY1 Monoclonal antibody (67856-1-Ig)

    WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCHY1 Monoclonal antibody (67856-1-Ig)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RCHY1 Monoclonal antibody (67856-1-Ig)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RCHY1 Monoclonal antibody (67856-1-Ig)

    IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RCHY1 Monoclonal antibody (67856-1-Ig)

    IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RCHY1 Monoclonal antibody, PBS Only

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67856-1-PBS
Clone No.2C11C11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67856-1-PBS targets RCHY1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RCHY1 fusion protein Ag4942 相同性解析による交差性が予測される生物種
Full Name ring finger and CHY zinc finger domain containing 1
Calculated molecular weight 261 aa, 30 kDa
GenBank accession numberBC047393
Gene Symbol RCHY1
Gene ID (NCBI) 25898
RRIDAB_2918614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PM5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.