Recoverin Polyclonal antibody
Recoverin Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 10073-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse eye tissue, rat retina tissue, Y79 cells, rat eye tissue |
Positive IF-P detected in | mouse eye tissue, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IHC | See 1 publications below |
IF | See 10 publications below |
Product Information
10073-1-AP targets Recoverin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recoverin fusion protein Ag0119 相同性解析による交差性が予測される生物種 |
Full Name | recoverin |
Calculated molecular weight | 24 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC001720 |
Gene symbol | Recoverin |
Gene ID (NCBI) | 5957 |
RRID | AB_2178005 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.
Protocols
Product Specific Protocols | |
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WB protocol for Recoverin antibody 10073-1-AP | Download protocol |
IF protocol for Recoverin antibody 10073-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
iScience Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease. | ||
iScience A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. | ||
iScience One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors. | ||
Sci Rep Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue. | ||
Sci Rep Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alessandro (Verified Customer) (07-27-2022) | No aspecific staining, great outcome
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