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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Recoverin Polyclonal antibody

Recoverin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10073

Synonyms

Protein CAR, RCV1, RCVRN, recoverin

Validation Data Gallery

Applications:
  • IF Staining of mouse eye using CL488-10073

    IF Staining of mouse eye using CL488-10073

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CoraLite® Plus 488 Recoverin antibody (CL488-10073) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inmouse eye tissue
For other applications, we recommend the unconjugated version of this antibody, 10073-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10073 targets Recoverin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recoverin fusion protein Ag0119 相同性解析による交差性が予測される生物種
Full Name recoverin
Calculated molecular weight 24 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001720
Gene symbol RCVRN
Gene ID (NCBI) 5957
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Recoverin antibody CL488-10073Download protocol
Standard Protocols
Click here to view our Standard Protocols