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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RD3 Polyclonal antibody, PBS Only

RD3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14855-1-PBS

Synonyms

Retinal degeneration protein 3, retinal degeneration 3, Protein RD3, LCA12, C1orf36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RD3 Polyclonal antibody (14855-1-AP)

    WB analysis using 14855-1-AP (same clone as 14855-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14855-1-AP (RD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RD3 Polyclonal antibody (14855-1-AP)

    IHC staining of mouse eye using 14855-1-AP (same clone as 14855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using RD3 Polyclonal antibody (14855-1-AP)

    IHC staining of rat eye using 14855-1-AP (same clone as 14855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using RD3 Polyclonal antibody (14855-1-AP)

    IHC staining of rat eye using 14855-1-AP (same clone as 14855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14855-1-PBS targets RD3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RD3 fusion protein Ag6641 相同性解析による交差性が予測される生物種
Full Name retinal degeneration 3
Calculated molecular weight22.7 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC065541
Gene symbol RD3
Gene ID (NCBI) 343035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RD3, or Retinal Degeneration 3, plays a critical role in regulating guanylate cyclase (GC) signaling and photoreceptor cell survival (PMID: 30559291). RD3 is highly conserved across vertebrates, with the human protein sharing high sequence identity with other primates and varying degrees of identity with other species (PMID: 29030614). The main functions of RD3 include inhibiting photoreceptor-specific guanylate cyclase activity and promoting the accumulation of retinal membrane guanylyl cyclase (RetGC) in the photoreceptor outer segment (PMID: 30559291). RD3 is essential for the normal expression of RetGC in photoreceptor cells and blocks RetGC catalytic activity. Mutations in the RD3 gene can lead to Leber congenital amaurosis type 12, which results in retinal degeneration. RD3 is also involved in the trafficking of RetGC from the endoplasmic reticulum to the photoreceptor outer segments, which is crucial for maintaining the normal function and survival of photoreceptors (PMID: 34537244).