Featured Product
KD/KO Validated

SLC1A5/ASCT2 Polyclonal antibody

Name: SLC1A5/ASCT2 Polyclonal antibody
Brand: Proteintech
SKU: 20350-1-AP

SLC1A5/ASCT2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20350-1-AP

Synonyms

SLC1A5, M7V1, Baboon M7 virus receptor, ATBO, ATB(0)

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis using 20350-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 20350-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 20350-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20350-1-AP (ASCT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 20350-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 20350-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20350-1-AP (ASCT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 20350-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using SLC1A5/ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta).
IF Staining of mouse small intestine using 20350-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta).
IF Staining of mouse small intestine using 20350-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta).
IF Staining of HEK-293 using 20350-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (20350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 20350-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (20350-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

-/ -

Cat No. 20350-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse small intestine tissue
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 21 publications below
IHC	See 6 publications below
IF	See 4 publications below

Product Information

20350-1-AP targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC1A5/ASCT2 fusion protein Ag14182 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 1 (neutral amino acid transporter), member 5
Calculated molecular weight	541 aa, 57 kDa
Observed molecular weight	55-70 kDa
GenBank accession number	BC000062
Gene symbol	SLC1A5/ASCT2
Gene ID (NCBI)	6510
RRID	AB_2878679
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).

Protocols

Product Specific Protocols
WB protocol for SLC1A5/ASCT2 antibody 20350-1-AP	Download protocol
IHC protocol for SLC1A5/ASCT2 antibody 20350-1-AP	Download protocol
IF protocol for SLC1A5/ASCT2 antibody 20350-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	ACS Nano Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer Authors - Lingtong Meng View Article
	WB	Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. Authors - Shuai Wang View Article
human	IHC	J Transl Med Elevated SLC1A5 associated with poor prognosis and therapeutic resistance to transarterial chemoembolization in hepatocellular carcinoma Authors - Guixiong Zhang View Article
human	WB	Cell Biol Toxicol Degradation of HIF-1α induced by curcumol blocks glutaminolysis and inhibits epithelial-mesenchymal transition and invasion in colorectal cancer cells. Authors - Yong Bian View Article
human	WB	Aging (Albany NY) The cross-talk between DDR1 and STAT3 promotes the development of hepatocellular carcinoma. Authors - Ye Lin View Article
human	WB	Mol Ther Oncolytics Improving the Efficacy of EGFR Inhibitors by Topical Treatment of Cutaneous Squamous Cell Carcinoma with miR-634 Ointment. Authors - Jun Inoue KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC1A5/ASCT2 Polyclonal antibody

SLC1A5/ASCT2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 20350-1-AP

IHC staining of human colon cancer using 20350-1-AP

IHC staining of human colon cancer using 20350-1-AP

IHC staining of human stomach cancer using 20350-1-AP

IHC staining of human stomach cancer using 20350-1-AP

IF Staining of mouse small intestine using 20350-1-AP

IF Staining of mouse small intestine using 20350-1-AP

IF Staining of mouse small intestine using 20350-1-AP

IF Staining of HEK-293 using 20350-1-AP

FC experiment of HeLa using 20350-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer

PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.

Elevated SLC1A5 associated with poor prognosis and therapeutic resistance to transarterial chemoembolization in hepatocellular carcinoma

Degradation of HIF-1α induced by curcumol blocks glutaminolysis and inhibits epithelial-mesenchymal transition and invasion in colorectal cancer cells.

The cross-talk between DDR1 and STAT3 promotes the development of hepatocellular carcinoma.

Improving the Efficacy of EGFR Inhibitors by Topical Treatment of Cutaneous Squamous Cell Carcinoma with miR-634 Ointment.