Validation Data Gallery
Filter:
Tested Applications
| Positive IF-P detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-20350 targets ASCT2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASCT2 fusion protein Ag14182 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 1 (neutral amino acid transporter), member 5 |
| Calculated molecular weight | 541 aa, 57 kDa |
| Observed molecular weight | 55-70 kDa |
| GenBank accession number | BC000062 |
| Gene Symbol | SLC1A5/ASCT2 |
| Gene ID (NCBI) | 6510 |
| RRID | AB_3084033 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15758 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ASCT2 antibody CL488-20350 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |