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KD/KO Validated

RECQL4 Polyclonal antibody

Name: RECQL4 Polyclonal antibody
Brand: Proteintech
SKU: 17008-1-AP

RECQL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17008-1-AP

Synonyms

RecQ protein like 4, EC:5.6.2.4, DNA helicase, RecQ-like type 4, DNA 3'-5' helicase RecQ4, ATP-dependent DNA helicase Q4

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 17008-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17008-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17008-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17008-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17008-1-AP
IP result of anti-RECQL4 (IP:17008-1-AP, 4ug; Detection:17008-1-AP 1:1000) with HeLa cells lysate 1880 ug.
IHC staining of human gliomas using 17008-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 17008-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 17008-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RECQL4 antibody (17008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 17008-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human gliomas tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

17008-1-AP targets RECQL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RECQL4 fusion protein Ag10707 相同性解析による交差性が予測される生物種
Full Name	RecQ protein-like 4
Calculated molecular weight	1208 aa, 133 kDa
Observed molecular weight	145-150 kDa
GenBank accession number	BC013277
Gene symbol	RECQL4
Gene ID (NCBI)	9401
RRID	AB_2238324
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

Protocols

Product Specific Protocols
WB protocol for RECQL4 antibody 17008-1-AP	Download protocol
IHC protocol for RECQL4 antibody 17008-1-AP	Download protocol
IF protocol for RECQL4 antibody 17008-1-AP	Download protocol
IP protocol for RECQL4 antibody 17008-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
human	IHC	Cancer Biol Med RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer. Authors - Guosheng Lyu KD Validated View Article
human	WB	FASEB J UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair. Authors - Qiuling Huang View Article
human	WB	J Biol Chem The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells. Authors - Jiewei Wang View Article
human	IF	Sci Rep PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner. Authors - Sebastian Veith View Article
human	IF	bioRxiv USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication Authors - Hannah L Mackay View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RECQL4 Polyclonal antibody

RECQL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 17008-1-AP

WB analysis of HeLa using 17008-1-AP

WB analysis of HeLa using 17008-1-AP

WB analysis of HeLa using 17008-1-AP

IP experiment of HeLa using 17008-1-AP

IHC staining of human gliomas using 17008-1-AP

IHC staining of human kidney using 17008-1-AP

IHC staining of human kidney using 17008-1-AP

IHC staining of human gliomas using 17008-1-AP

IF Staining of HeLa using 17008-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.

UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair.

The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.

PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.

USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication