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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RECQL4 Polyclonal antibody

RECQL4 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17008

Synonyms

ATP dependent DNA helicase Q4, DNA helicase, RecQ like type 4, RecQ protein like 4, RECQ4, RECQL4, RTS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RECQL4 Polyclonal an (CL488-17008)

    IF Staining of HeLa using CL488-17008

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RECQL4 antibody (CL488-17008) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17008 targets RECQL4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RECQL4 fusion protein Ag10707 相同性解析による交差性が予測される生物種
Full Name RecQ protein-like 4
Calculated molecular weight 1208 aa, 133 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC013277
Gene symbol RECQL4
Gene ID (NCBI) 9401
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RECQL4 antibody CL488-17008Download protocol
Standard Protocols
Click here to view our Standard Protocols