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REEP5 Monoclonal antibody

REEP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H8D8

Cat no : 68119-1-Ig

Synonyms

C5orf18, D5S346, DP1, Polyposis locus protein 1, Protein TB2, receptor accessory protein 5, REEP5, TB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REEP5 Monoclonal antibody (68119-1-Ig)

    WB analysis using 68119-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using REEP5 Monoclonal antibody (68119-1-Ig)

    WB analysis of HeLa using 68119-1-Ig

    WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.

  • Western Blot (WB) analysis of pig heart tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    WB analysis of pig heart using 68119-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    IHC staining of human colon cancer using 68119-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    IHC staining of human liver using 68119-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using REEP5 Monoclonal antibody (68119-1-Ig)

    IHC staining of human liver using 68119-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using REEP5 Monoclonal antibody (68119-1-Ig)

    IF Staining of A549 using 68119-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, pig heart tissue, HeLa cells, LNCaP cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68119-1-Ig targets REEP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen REEP5 fusion protein Ag6229 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 5
Calculated molecular weight 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC065926
Gene symbol REEP5
Gene ID (NCBI) 7905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
WB protocol for REEP5 antibody 68119-1-IgDownload protocol
IHC protocol for REEP5 antibody 68119-1-IgDownload protocol
IF protocol for REEP5 antibody 68119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols