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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated REEP5 Monoclonal antibody

REEP5 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8D8

Cat no : CL488-68119

Synonyms

C5orf18, D5S346, DP1, Polyposis locus protein 1, Protein TB2, receptor accessory protein 5, REEP5, TB2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated REEP5 Monoclonal ant (CL488-68119)

    IF Staining of A549 using CL488-68119

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 REEP5 antibody (CL488-68119, Clone: 1H8D8 ) at dilution of 1:200.

Tested Applications

Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68119 targets REEP5 in IF applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen REEP5 fusion protein Ag6229 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 5
Calculated molecular weight 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC065926
Gene symbol REEP5
Gene ID (NCBI) 7905
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 REEP5 antibody CL488-68119Download protocol
FC protocol for CL Plus 488 REEP5 antibody CL488-68119Download protocol
Standard Protocols
Click here to view our Standard Protocols