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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated REEP5 Monoclonal antibody

REEP5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8D8

Cat no : CL488-68119

Synonyms

Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1, C5orf18

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated REEP5 Monoclonal ant (CL488-68119)

    IF Staining of A549 using CL488-68119

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 REEP5 antibody (CL488-68119, Clone: 1H8D8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated REEP5 Monoclonal ant (CL488-68119)

    FC experiment of HeLa using CL488-68119

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human REEP5 (CL488-68119, Clone:1H8D8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68119 targets REEP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen REEP5 fusion protein Ag6229 相同性解析による交差性が予測される生物種
Full Name receptor accessory protein 5
Calculated molecular weight 21 kDa
Observed molecular weight18 kDa
GenBank accession numberBC065926
Gene symbol REEP5
Gene ID (NCBI) 7905
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 REEP5 antibody CL488-68119Download protocol
Standard Protocols
Click here to view our Standard Protocols