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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RELB Polyclonal antibody

RELB Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25027

Synonyms

Transcription factor RelB, I-Rel

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RELB Polyclonal anti (CL488-25027)

    IF Staining of HeLa using CL488-25027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RELB antibody (CL488-25027) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated RELB Polyclonal anti (CL488-25027)

    FC experiment of Jurkat using CL488-25027

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RELB (CL488-25027) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25027 targets RELB in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RELB fusion protein Ag13824 相同性解析による交差性が予測される生物種
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Calculated molecular weight 62 kDa
Observed molecular weight 62-65 kDa
GenBank accession numberBC028013
Gene symbol RELB
Gene ID (NCBI) 5971
RRIDAB_2919201
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RELB antibody CL488-25027Download protocol
Standard Protocols
Click here to view our Standard Protocols