Validation Data Gallery
Tested Applications
| Positive WB detected in | OS-RC-2 cells, A431 cells, MG-63 cells, ACHN cells, Caki-1 cells |
| Positive IHC detected in | human kidney tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
60128-1-Ig targets RENALASE in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RENALASE fusion protein Ag13061 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 10 open reading frame 59 |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC005364 |
| Gene Symbol | RENALASE |
| Gene ID (NCBI) | 55328 |
| RRID | AB_2034903 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q5VYX0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RENALASE antibody 60128-1-Ig | Download protocol |
| IHC protocol for RENALASE antibody 60128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Am Soc Hypertens Human renalase: a review of its biology, function, and implications for hypertension. | ||
bioRxiv Renalase inhibition regulates β cell metabolism to defend against acute and chronic stress |