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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RENALASE Monoclonal antibody (60128-1-Ig)

    WB analysis using 60128-1-Ig (same clone as 60128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RENALASE Monoclonal antibody (60128-1-Ig)

    WB analysis using 60128-1-Ig (same clone as 60128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MG-63 cells using RENALASE Monoclonal antibody (60128-1-Ig)

    WB analysis of MG-63 using 60128-1-Ig (same clone as 60128-1-PBS)

    MG-63 cells were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RENALASE Monoclonal antibody (60128-1-Ig)

    IHC staining of human kidney using 60128-1-Ig (same clone as 60128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RENALASE Monoclonal antibody (60128-1-Ig)

    IHC staining of human kidney using 60128-1-Ig (same clone as 60128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using RENALASE Monoclonal antibody (60128-1-Ig)

    IHC staining of human skeletal muscle using 60128-1-Ig (same clone as 60128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using RENALASE Monoclonal antibody (60128-1-Ig)

    IHC staining of human skeletal muscle using 60128-1-Ig (same clone as 60128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

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RENALASE Monoclonal antibody, PBS Only

RENALASE Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60128-1-PBS
Clone No.3A12F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

C10orf59, 3A12F5, Monoamine oxidase C, MAO-C, MAO C

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60128-1-PBS targets RENALASE in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RENALASE fusion protein Ag13061 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 59
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005364
Gene Symbol RENALASE
Gene ID (NCBI) 55328
RRIDAB_2034903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ5VYX0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.