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- KD/KO Validated
CoraLite® Plus 488-conjugated REST Polyclonal antibody
REST Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-22242
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-22242 targets REST in IF/ICC applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | REST fusion protein Ag17707 相同性解析による交差性が予測される生物種 |
| Full Name | RE1-silencing transcription factor |
| Calculated molecular weight | 1097 aa, 122 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC132859 |
| Gene symbol | REST |
| Gene ID (NCBI) | 5978 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 REST antibody CL488-22242 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
