RFC3 Monoclonal antibody
RFC3 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G3A8
Cat no : 68290-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68290-1-Ig targets RFC3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | RFC3 fusion protein Ag28365 相同性解析による交差性が予測される生物種 |
Full Name | replication factor C (activator 1) 3, 38kDa |
Calculated molecular weight | 40 aa, 6 kDa |
Observed molecular weight | 38 kDa |
GenBank accession number | BC000149 |
Gene symbol | RFC3 |
Gene ID (NCBI) | 5983 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for RFC3 antibody 68290-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |