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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFC3 Monoclonal antibody (68290-1-Ig)

    WB analysis using 68290-1-Ig (same clone as 68290-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RFC3 Monoclonal antibody (68290-1-Ig)

    WB analysis of NIH/3T3 using 68290-1-Ig (same clone as 68290-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using RFC3 Monoclonal antibody (68290-1-Ig)

    WB analysis of HSC-T6 using 68290-1-Ig (same clone as 68290-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

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RFC3 Monoclonal antibody, PBS Only

RFC3 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68290-1-PBS
Clone No.2G3A8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68290-1-PBS targets RFC3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RFC3 fusion protein Ag28365 相同性解析による交差性が予測される生物種
Full Name replication factor C (activator 1) 3, 38kDa
Calculated molecular weight 40 aa, 6 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000149
Gene Symbol RFC3
Gene ID (NCBI) 5983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40938
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.