Validation Data Gallery
Product Information
The ChromoTek RFP-Trap® Magnetic Agarose are affinity beads for IP of RFP-fusion proteins. It comprises a RFP Nanobody/ VHH coupled to magnetic agarose.
| Description | Immunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to magnetic agarose beads..
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 22.5 μg of recombinant RFP per 25 μL bead slurry |
| Conjugate | Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 5 nM |
| Compatibility with mass spectrometry | The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631363 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
|---|
| rtma_SDS_RFP-Trap® Magnetic Agarose (EN) |
| Datasheet |
|---|
| RFP-Trap® Magnetic Agarose Datasheet |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (IP) (PDF) |
| White papers |
|---|
| Split Fluorescent Protein Technology (PDF) |
| RFP-Trap Specificity |
|---|
| Fluorescent protein specificity table (PDF) |
| Application notes |
|---|
| Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins |
Publications
| Application | Title |
|---|---|
Cell Chromatin remodeling of histone H3 variants by DDM1 underlies epigenetic inheritance of DNA methylation | |
Nature POLAR-guided signalling complex assembly and localization drive asymmetric cell division. | |
Cell Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation. | |
Nat Struct Mol Biol SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity. | |
Nat Cell Biol Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. |