Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells, U2OS cells, K-562 cells, A549 cells, MCF-7 cells, LnCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68057-1-Ig targets RFX1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RFX1 fusion protein Ag25442 相同性解析による交差性が予測される生物種 |
| Full Name | regulatory factor X, 1 (influences HLA class II expression) |
| Calculated molecular weight | 105 kDa |
| Observed molecular weight | 135 kDa |
| GenBank accession number | BC049826 |
| Gene Symbol | RFX1 |
| Gene ID (NCBI) | 5989 |
| RRID | AB_2918798 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RFX1 antibody 68057-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |