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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFX1 Monoclonal antibody, PBS Only

RFX1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10H6

Cat no : 68057-1-PBS

Synonyms

RFX, EF-C, EF C, 1G10H6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis of U2OS using 68057-1-Ig (same clone as 68057-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis of HeLa using 68057-1-Ig (same clone as 68057-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis of NIH/3T3 using 68057-1-Ig (same clone as 68057-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68057-1-PBS targets RFX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RFX1 fusion protein Ag25442 相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 1 (influences HLA class II expression)
Calculated molecular weight 105 kDa
Observed molecular weight135 kDa
GenBank accession numberBC049826
Gene symbol RFX1
Gene ID (NCBI) 5989
RRIDAB_2918798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).