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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS14 Monoclonal antibody

RGS14 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 67394-1-Ig

Synonyms

Regulator of G-protein signaling 14, 1C1B2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of mouse brain using 67394-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of HeLa using 67394-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of HEK-293 using 67394-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of Jurkat using 67394-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of NIH/3T3 using 67394-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RGS14 Monoclonal antibody (67394-1-Ig)

    IHC staining of mouse brain using 67394-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RGS14 Monoclonal antibody (67394-1-Ig)

    IHC staining of mouse brain using 67394-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RGS14 Monoclonal antibody (67394-1-Ig)

    IF Staining of HeLa using 67394-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using RGS14 Monoclonal antibody (67394-1-Ig)

    FC experiment of HeLa using 67394-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:15000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67394-1-Ig targets RGS14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RGS14 fusion protein Ag9477 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 14
Calculated molecular weight 566 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014094
Gene symbol RGS14
Gene ID (NCBI) 10636
RRIDAB_2882638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.

Protocols

Product Specific Protocols
WB protocol for RGS14 antibody 67394-1-IgDownload protocol
IHC protocol for RGS14 antibody 67394-1-IgDownload protocol
IF protocol for RGS14 antibody 67394-1-IgDownload protocol
FC protocol for RGS14 antibody 67394-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols