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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of HEK-293 using 67394-1-Ig (same clone as 67394-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of Jurkat using 67394-1-Ig (same clone as 67394-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RGS14 Monoclonal antibody (67394-1-Ig)

    WB analysis of NIH/3T3 using 67394-1-Ig (same clone as 67394-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RGS14 Monoclonal antibody (67394-1-Ig)

    IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RGS14 Monoclonal antibody (67394-1-Ig)

    IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RGS14 Monoclonal antibody (67394-1-Ig)

    IF Staining of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RGS14 Monoclonal antibody (67394-1-Ig)

    FC experiment of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

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RGS14 Monoclonal antibody, PBS Only

RGS14 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67394-1-PBS
Clone No.1C1B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Regulator of G-protein signaling 14, 1C1B2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67394-1-PBS targets RGS14 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RGS14 fusion protein Ag9477 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 14
Calculated molecular weight 566 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014094
Gene Symbol RGS14
Gene ID (NCBI) 10636
RRIDAB_2882638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43566
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.