Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, C6 cells, Raji cells, SH-SY5Y cells, U-251 cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
66441-1-Ig targets RGS4 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Reactivity | human, rat, mouse, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RGS4 fusion protein Ag6029 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 4 |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC051869 |
| Gene Symbol | RGS4 |
| Gene ID (NCBI) | 5999 |
| RRID | AB_2881811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49798 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS4 antibody 66441-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hippocampus NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus. | ||
J Cell Physiol MEOX1 triggers myofibroblast apoptosis resistance, contributing to pulmonary fibrosis in mice |