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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS4 Monoclonal antibody, PBS Only

RGS4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2E2

Cat no : 66441-1-PBS

Synonyms

RGP4, RGS4, SCZD9

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Neuro-2a cells using RGS4 Monoclonal antibody (66441-1-Ig)

    WB analysis of Neuro-2a using 66441-1-Ig (same clone as 66441-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using RGS4 Monoclonal antibody (66441-1-Ig)

    WB analysis of C6 using 66441-1-Ig (same clone as 66441-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using RGS4 Monoclonal antibody (66441-1-Ig)

    WB analysis of Y79 using 66441-1-Ig (same clone as 66441-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using RGS4 Monoclonal antibody (66441-1-Ig)

    WB analysis of SH-SY5Y using 66441-1-Ig (same clone as 66441-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using RGS4 Monoclonal antibody (66441-1-Ig)

    WB analysis of U-251 using 66441-1-Ig (same clone as 66441-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using RGS4 Monoclonal antibody (66441-1-Ig)

    WB analysis of Raji using 66441-1-Ig (same clone as 66441-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66441-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66441-1-PBS targets RGS4 in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RGS4 fusion protein Ag6029 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 4
Calculated molecular weight 23 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC051869
Gene symbol RGS4
Gene ID (NCBI) 5999
RRIDAB_2881811
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.