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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RGS5 Polyclonal antibody

RGS5 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11590

Synonyms

Regulator of G-protein signaling 5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HT-1080 cells using CoraLite® Plus 488-conjugated RGS5 Polyclonal anti (CL488-11590)

    IF Staining of HT-1080 using CL488-11590

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-1080 cells using CoraLite® Plus 488 RGS5 antibody (CL488-11590) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RGS5 Polyclonal anti (CL488-11590)

    FC experiment of HeLa using CL488-11590

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RGS5 (CL488-11590) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHT-1080 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11590 targets RGS5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS5 fusion protein Ag2196 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 5
Calculated molecular weight 181 aa, 21 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC030059
Gene symbol RGS5
Gene ID (NCBI) 8490
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RGS5 antibody CL488-11590Download protocol
FC protocol for CL Plus 488 RGS5 antibody CL488-11590Download protocol
Standard Protocols
Click here to view our Standard Protocols