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- KD/KO Validated
RHOA Monoclonal antibody
RHOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, pig, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1B3D7
Cat no : 66733-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
66733-1-Ig targets RHOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| Tested Reactivity | Human, mouse, pig, rat |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RHOA fusion protein Ag1141 相同性解析による交差性が予測される生物種 |
| Full Name | ras homolog gene family, member A |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC005976 |
| Gene symbol | RHOA |
| Gene ID (NCBI) | 387 |
| RRID | AB_2882083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RHOA antibody 66733-1-Ig | Download protocol |
| IHC protocol for RHOA antibody 66733-1-Ig | Download protocol |
| IF protocol for RHOA antibody 66733-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserines. | ||
Clin Sci (Lond) The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling. | ||
J Biol Chem SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion
| ||
Front Mol Biosci CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma. | ||
J Cancer GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer. | ||
Exp Cell Res RALY may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (12-22-2023) | Worked well for WB in Glioma cells at 1:1000 in BSA
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