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  • Featured Product
  • KD/KO Validated

RHOA Monoclonal antibody, PBS Only

RHOA Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D7

Cat no : 66733-1-PBS

Synonyms

ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOA Monoclonal antibody (66733-1-Ig)

    WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOA Monoclonal antibody (66733-1-Ig)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOA Monoclonal antibody (66733-1-Ig)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOA Monoclonal antibody (66733-1-Ig)

    IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66733-1-PBS targets RHOA in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOA fusion protein Ag1141 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member A
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.