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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RHOC Monoclonal antibody

RHOC Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1C1

Cat no : CL488-67542

Synonyms

ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RHOC Monoclonal anti (CL488-67542)

    IF Staining of HeLa using CL488-67542

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RHOC antibody (CL488-67542, Clone: 1G1C1 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67542 targets RHOC in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOC fusion protein Ag14633 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member C
Calculated molecular weight 22 kDa
GenBank accession numberBC007245
Gene symbol RHOC
Gene ID (NCBI) 389
RRIDAB_2919497
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RHOC antibody CL488-67542Download protocol
Standard Protocols
Click here to view our Standard Protocols