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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis of Raji using 67143-1-Ig (same clone as 67143-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis of mouse brain using 67143-1-Ig (same clone as 67143-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis of HL-60 using 67143-1-Ig (same clone as 67143-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RIAM Monoclonal antibody (67143-1-Ig)

    IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RIAM Monoclonal antibody (67143-1-Ig)

    IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using RIAM Monoclonal antibody (67143-1-Ig)

    IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using RIAM,APBB1IP antibody (67143-1-Ig, Clone: 2F8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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RIAM Monoclonal antibody, PBS Only

RIAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67143-1-PBS
Clone No.2F8A2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67143-1-PBS targets RIAM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RIAM fusion protein Ag4602 相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
Calculated molecular weight 666 aa, 73 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC035636
Gene Symbol RIAM
Gene ID (NCBI) 54518
RRIDAB_2882442
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z5R6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.