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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIAM Monoclonal antibody

RIAM Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F8A2

Cat no : 67143-1-Ig

Synonyms

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis using 67143-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis of Raji using 67143-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis of mouse brain using 67143-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using RIAM Monoclonal antibody (67143-1-Ig)

    WB analysis of HL-60 using 67143-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RIAM Monoclonal antibody (67143-1-Ig)

    IHC staining of human tonsillitis using 67143-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RIAM Monoclonal antibody (67143-1-Ig)

    IHC staining of human tonsillitis using 67143-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using RIAM Monoclonal antibody (67143-1-Ig)

    IF Staining of human tonsillitis using 67143-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using RIAM,APBB1IP antibody (67143-1-Ig, Clone: 2F8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, Raji cells, mouse brain tissue, Jurkat cells, Ramos cells, Dauli cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67143-1-Ig targets RIAM in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RIAM fusion protein Ag4602 相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
Calculated molecular weight 666 aa, 73 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC035636
Gene symbol RIAM
Gene ID (NCBI) 54518
RRIDAB_2882442
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.

Protocols

Product Specific Protocols
WB protocol for RIAM antibody 67143-1-IgDownload protocol
IHC protocol for RIAM antibody 67143-1-IgDownload protocol
IF protocol for RIAM antibody 67143-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols