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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RILPL1 Recombinant antibody (83220-6-RR)

    WB analysis using 83220-6-RR (same clone as 83220-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83220-6-RR (RILPL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83220-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RILPL1 Recombinant antibody (83220-6-RR)

    WB analysis of HepG2 using 83220-6-RR (same clone as 83220-6-PBS)

    WB result of RILPL1 antibody (83220-6-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HepG2 cells. This data was developed using the same antibody clone with 83220-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83220-6-RR

    Affinity and Kinetic Characterization of 83220-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83220-6-RR against Human RILPL1 were performed. The affinity constant is 1.97 nM.

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  • KD/KO Validated

RILPL1 Recombinant antibody, PBS Only

RILPL1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83220-6-PBS
Clone No.230515D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83220-6-PBS targets RILPL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RILPL1 fusion protein Ag10192 相同性解析による交差性が予測される生物種
Full Name Rab interacting lysosomal protein-like 1
Calculated molecular weight 252 aa, 29 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC080626
Gene Symbol RILPL1
Gene ID (NCBI) 353116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5EBL4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein localization in the primary cilium. Rilpl2 was initially identified as up-regulated in ciliating mouse tracheal epithelial cells. Rilpl1 and Rilpl2 both localize to the primary cilium and centrosome, Rilpl1 specifically to the distal end of the mother centriole.(PMID:23264467)