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RILPL1 Recombinant antibody

RILPL1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230515D1

Cat no : 83220-6-RR

Synonyms

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RILPL1 Recombinant antibody (83220-6-RR)

    WB analysis using 83220-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83220-6-RR (RILPL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RILPL1 Recombinant antibody (83220-6-RR)

    WB analysis of HepG2 using 83220-6-RR

    WB result of RILPL1 antibody (83220-6-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 83220-6-RR

    Affinity and Kinetic Characterization of 83220-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83220-6-RR against Human RILPL1 were performed. The affinity constant is 1.97 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, A549 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83220-6-RR is RILPL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RILPL1 fusion protein Ag10192 相同性解析による交差性が予測される生物種
Full Name Rab interacting lysosomal protein-like 1
Calculated molecular weight 252 aa, 29 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC080626
Gene symbol RILPL1
Gene ID (NCBI) 353116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein localization in the primary cilium. Rilpl2 was initially identified as up-regulated in ciliating mouse tracheal epithelial cells. Rilpl1 and Rilpl2 both localize to the primary cilium and centrosome, Rilpl1 specifically to the distal end of the mother centriole.(PMID:23264467)

Protocols

Product Specific Protocols
WB protocol for RILPL1 antibody 83220-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols