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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using CoraLite® Plus 488-conjugated RLBP1 Polyclonal ant (CL488-15356)

    IF Staining of mouse eye using CL488-15356

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CoraLite® Plus 488 RLBP1 antibody (CL488-15356) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using CoraLite® Plus 488-conjugated RLBP1 Polyclonal ant (CL488-15356)

    IF Staining of mouse eye using CL488-15356

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CoraLite® Plus 488 RLBP1 antibody (CL488-15356) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CoraLite® Plus 488-conjugated RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for IF-P
Cat No. CL488-15356

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

CRALBP, RLBP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15356 targets RLBP1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RLBP1 fusion protein Ag7602 相同性解析による交差性が予測される生物種
Full Name retinaldehyde binding protein 1
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004199
Gene Symbol RLBP1
Gene ID (NCBI) 6017
RRIDAB_3672630
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12271
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RLBP1 antibody CL488-15356Download protocol
Standard Protocols
Click here to view our Standard Protocols